Anticorps Monoclonal anti-SRY

SRY Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E9A4

N° de cat : 67349-1-PBS

Synonymes

2E9A4, sex determining region Y, Sex-determining region Y protein, Testis-determining factor

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Galerie de données de validation

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  WB analysis of LNCaP using 67349-1-Ig (same clone as 67349-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

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  WB analysis using 67349-1-Ig (same clone as 67349-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 67349-1-Ig (same clone as 67349-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67349-1-PBS cible SRY dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SRY Protéine recombinante Ag12610
• Nom complet: sex determining region Y
• Masse moléculaire calculée: 204 aa, 24 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC074924
• Symbole du gène: SRY
• Identification du gène (NCBI): 6736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.

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