Anticorps Polyclonal de lapin anti-TRAPA/SSR1

• Phare
• Validé par KD/KO

TRAPA/SSR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 10583-1-AP

Synonymes

SSR1, TRAP alpha, PSEC0262, SSR alpha, SSR-alpha

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Galerie de données de validation

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  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10583-1-AP

  WB result of TRAPA/SSR1 antibody (10583-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPA/SSR1 transfected HepG2 cells.

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  WB analysis of human liver using 10583-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10583-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10583-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TRAPA/SSR1 antibody (10583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules COS-7, cellules HepG2, cellules L02, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

10583-1-AP cible TRAPA/SSR1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Réactivité citée: rat, Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAPA/SSR1 Protéine recombinante Ag0878
• Nom complet: signal sequence receptor, alpha
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC007710
• Symbole du gène: TRAPA
• Identification du gène (NCBI): 6745
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Publications

Species	Application	Title
human	IF	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	IF	Biosens Bioelectron; Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system.; Authors - Mao Chen
human	WB	FASEB J; GPR37 is processed in the N-terminal ectodomain by ADAM10 and furin.; Authors - S Orvokki Mattila
rat	WB,IF	Exp Cell Res; Microdomains of endoplasmic reticulum within the sarcoplasmic reticulum of skeletal myofibers.; Authors - Kaakinen Mika M

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chatchai (Verified Customer) (09-26-2019)

This anti-SSR1 is very good, presents the specific band at the right size of the protein.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:500 for WB
• Cell Tissue Type: A549, HEK-293

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