Anticorps Monoclonal anti-SSSCA1

SSSCA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D2E8

N° de cat : 68423-1-PBS

Synonymes

Autoantigen p27, p27, SSSCA1, ZNRD2

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Galerie de données de validation

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  WB analysis using 68423-1-Ig (same clone as 68423-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68423-1-Ig (same clone as 68423-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68423-1-PBS cible SSSCA1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SSSCA1 Protéine recombinante Ag16783
• Nom complet: Sjogren syndrome/scleroderma autoantigen 1
• Masse moléculaire calculée: 199 aa, 21 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC014791
• Symbole du gène: SSSCA1
• Identification du gène (NCBI): 10534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.

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