Anticorps Polyclonal de lapin anti-Somatostatin (64-81aa)

Somatostatin (64-81aa) Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 17512-1-AP

Synonymes

SMST, somatostatin, Somatostatin (64-81aa), SST

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Galerie de données de validation

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  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:25600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:25600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP Somatostatin-Specific antibody (green) and 66198-1-Ig Insulin antibody (red) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu pancréatique de souris, tissu cérébral de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pancréatique humain, tissu cérébral de souris, tissu pancréatique de souris

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:6400-1:25600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

17512-1-AP cible Somatostatin (64-81aa) dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: somatostatin
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: NM_001048
• Symbole du gène: SST
• Identification du gène (NCBI): 6750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. This antibody can recognize the full length and propeptide of Somatostatin.

Publications

Species	Application	Title
rat	IF	Aging Dis; PMID: 31440379; Authors - Fei Han
	IF	Cell Death Dis; Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis.; Authors - Xiaoyi Zhang