Anticorps Recombinant de lapin anti-Somatostatin

Somatostatin Recombinant Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240816D9

N° de cat : 84351-1-PBS

Synonymes

SST, somatostatin, SST, SMST, Growth hormone release-inhibiting factor, 240816D9

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Galerie de données de validation

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  IHC staining of mouse pancreas using 84351-1-RR (same clone as 84351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-RR (SST-Specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84351-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 84351-1-PBS

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-PBS (Somatostatin antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 84351-1-RR (same clone as 84351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-RR (SST-Specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84351-1-PBS

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-PBS (Somatostatin antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 84351-1-RR (same clone as 84351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Somatostatin antibody (84351-1-RR, Clone: 240816D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84351-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 84351-1-RR (same clone as 84351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Somatostatin antibody (84351-1-RR, Clone: 240816D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84351-1-PBS in a different storage buffer formulation.

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Applications testées

• Résultats positifs en IHC: tissu pancréatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20000-1:80000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84351-1-PBS cible Somatostatin dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: somatostatin
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: NM_001048
• Symbole du gène: Somatostatin
• Identification du gène (NCBI): 6750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids.

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