Anticorps Polyclonal de lapin anti-SSU72

SSU72 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15434-1-AP

Synonymes

RNA polymerase II subunit A C-terminal domain phosphatase SSU72, PNAS-120, PNAS 120, HSPC182, EC:3.1.3.16

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Galerie de données de validation

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  WB analysis using 15434-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 15434-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15434-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer de la prostate humain, tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15434-1-AP cible SSU72 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SSU72 Protéine recombinante Ag7699
• Nom complet: SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC008070
• Symbole du gène: SSU72
• Identification du gène (NCBI): 29101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Sci Adv; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency; Authors - Chikako Okubo
		Mol Cell; Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors; Authors - Chengjiang Lu

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