Anticorps Polyclonal de lapin anti-ST3GAL4

ST3GAL4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 13546-1-AP

Synonymes

Alpha 2,3-sialyltransferase IV, Alpha 2,3-ST 4, Beta-galactoside alpha-2,3-sialyltransferase 4, CGS23, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4

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Galerie de données de validation

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  WB analysis using 13546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13546-1-AP (ST3GAL4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13546-1-AP (ST3GAL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13546-1-AP (ST3GAL4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13546-1-AP (ST3GAL4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13546-1-AP (ST3GAL4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13546-1-AP (ST3GAL4 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HCT 116, cellules HepG2, cellules MCF-7, cellules MDA-MB-231
• Résultats positifs en IHC: tissu testiculaire humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

13546-1-AP cible ST3GAL4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ST3GAL4 Protéine recombinante Ag3665
• Nom complet: ST3 beta-galactoside alpha-2,3-sialyltransferase 4
• Masse moléculaire calculée: 333 aa, 38 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC010645
• Symbole du gène: ST3GAL4
• Identification du gène (NCBI): 6484
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ST3GAL4, also known as CGS23, NANTA3 and SIAT4C, is a member of the glycosyltransferase 29 family. ST3GAL4 protein exists in multiple splice variants and is frequently modified by glycosylation. ST3GAL4 is expressed uniformly in tissues and organs during all stages of development, and the subcellular localization is currently not clear.

Publications

Species	Application	Title
human	WB,IHC	Front Oncol; ST3Gal IV Mediates the Growth and Proliferation of Cervical Cancer Cells In Vitro and In Vivo Via the Notch/p21/CDKs Pathway.; Authors - Yinshuang Wu
human	WB,IHC	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu
human	WB,IHC	FEBS Lett; The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.; Authors - Ming Yu
human	WB	Glycobiology; Sialylation-dependent interaction between PD-L1 and CD169 promotes monocytes adhesion to endothelial cells; Authors - Kebo Cai
mouse	IF	Elife; RANK+TLR2+ myeloid subpopulation converts autoimmune to joint destruction in rheumatoid arthritis; Authors - Weixin Zhang
human	IHC	Hum Cell; ST3GAL4 promotes tumorigenesis in breast cancer by enhancing aerobic glycolysis; Authors - Xiaoqing Chen

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