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Anticorps Polyclonal de lapin anti-ST3GAL5

ST3GAL5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30120-1-PBS

Synonymes

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using ST3GAL5 Polyclonal antibody (30120-1-AP)

    WB analysis using 30120-1-AP (same clone as 30120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30120-1-AP (ST3GAL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ST3GAL5 Polyclonal antibody (30120-1-AP)

    IF Staining of U2OS using 30120-1-AP (same clone as 30120-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ST3GAL5 antibody (30120-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ST3GAL5 Polyclonal antibody (30120-1-AP)

    IF Staining of U2OS using 30120-1-AP (same clone as 30120-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ST3GAL5 antibody (30120-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

Informations sur le produit

30120-1-PBS cible ST3GAL5 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ST3GAL5 Protéine recombinante Ag32461
Nom complet ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Masse moléculaire calculée 48 kDa
Poids moléculaire observé 70 kDa
Numéro d’acquisition GenBankBC065936
Symbole du gène ST3GAL5
Identification du gène (NCBI) 8869
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is high expression in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). This antibody recognizes the glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).

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