Anticorps Polyclonal de lapin anti-ST8SIA2-Specific

ST8SIA2-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 19736-1-AP

Synonymes

Alpha 2,8 sialyltransferase 8B, HsT19690, Sialyltransferase 8B, Sialyltransferase X, Sialytransferase St8Sia II, SIAT8 B, SIAT8B, ST8SIA II, ST8SIA2, ST8SIA2-Specific, ST8SiaII, STX

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Galerie de données de validation

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  WB analysis of mouse heart using 19736-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19736-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 19736-1-AP (ST8SIA2-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 19736-1-AP (ST8SIA2-Specific Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

19736-1-AP cible ST8SIA2-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_006011
• Symbole du gène: ST8SIA2
• Identification du gène (NCBI): 8128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ST8SIA2, also named as SIAT8B and STX, belongs to the glycosyltransferase 29 family. ST8SIA2 may transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression. The antibody is specific to ST8SIA2.

Publications

Species	Application	Title
mouse	IF	Nat Neurosci; Single-cell genomics of the mouse olfactory cortex reveals contrasts with neocortex and ancestral signatures of cell type evolution; Authors - Sara Zeppilli
mouse,rat	WB,IHC	Oxid Med Cell Longev; Long Noncoding RNA TUG1 Aggravates Cerebral Ischemia/Reperfusion Injury by Acting as a ceRNA for miR-3072-3p to Target St8sia2.; Authors - Miao Chen
human	WB,IF	J Cell Sci; Prolyl endopeptidase is involved in the degradation of neural cell adhesion molecules in vitro.; Authors - Külli Jaako
mouse	WB	Brain Res; PSA modification of NCAM supports the survival of injured retinal ganglion cells in adulthood.; Authors - Natalia Lobanovskaya
human	IHC	Front Immunol; A novel ganglioside-related risk signature can reveal the distinct immune landscape of neuroblastoma and predict the immunotherapeutic response; Authors - Jiaxing Yang

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