Anticorps Recombinant de lapin anti-STAMBP

STAMBP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251545B3

N° de cat : 86601-3-RR

Synonymes

AMSH, EC:3.4.19.-, Endosome-associated ubiquitin isopeptidase, STAM binding protein, STAM-binding protein

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Galerie de données de validation

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  WB analysis using 86601-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86601-3-RR (STAMBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86601-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86601-3-RR (STAMBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 86601-3-RR

  Rat brain tissue was subjected to SDS PAGE followed by western blot with 86601-3-RR (STAMBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 86601-3-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using STAMBP antibody (86601-3-RR, Clone: 251545B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86601-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86601-3-RR against Human STAMBP were performed. The affinity constant is 15.2 pM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules SW480, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86601-3-RR cible STAMBP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: STAMBP Protéine recombinante Ag1895
• Nom complet: STAM binding protein
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC007682
• Symbole du gène: STAMBP
• Identification du gène (NCBI): 10617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAM-binding protein (STAMBP) is also named AMSH. STAMBP, a member of the Jab1/MPN metalloenzyme family of deubiquitinating enzymes (DUBs), specifically cleaves K63-linked polyubiquitination chains from substrates (PMID: 15314065, PMID: 26601948). STAMBP is implicated in the endosome-lysosome pathway and shuttles ubiquitinated substrate proteins to lysosomes for degradation (PMID: 10383417, PMID: 24151880). STAMBP regulates protein degradation by binding to the ESCRT-0 protein STAM and the charged multivesicular body proteins of the ESCRT-III complex (PMID: 20159979). STAMBP is implicated in the inflammatory response by promoting the deubiquitination and stabilization of NALP7 and increasing the activity of inflammasomes (PMID: 28492230). STAMBP expression was increased in the cytoplasm of tumor cells from LUAD patients. STAMBP overexpression promoted cell migration and invasion, whereas STAMBP knockdown attenuated these processes in LUAD cells after epidermal growth factor treatment (PMID: 3410245).

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