Anticorps Polyclonal de lapin anti-STAMBPL1

STAMBPL1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 27315-1-AP

Synonymes

ALMalpha, AMSH FP, AMSH like protease, AMSH LP, AMSHLP, KIAA1373, STAM binding protein like 1, STAMBPL1

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Galerie de données de validation

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  WB analysis of human testis using 27315-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27315-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27315-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 27315-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules MCF-7, tissu testiculaire humain
• Résultats positifs en IHC: tissu hépatique humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

27315-1-AP cible STAMBPL1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAMBPL1 Protéine recombinante Ag26308
• Nom complet: STAM binding protein-like 1
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC010846
• Symbole du gène: STAMBPL1
• Identification du gène (NCBI): 57559
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Phytomedicine; A multi-omic analysis reveals that Gamabufotalin exerts anti-hepatocellular carcinoma effects by regulating amino acid metabolism through targeting STAMBPL1; Authors - Piao Zheng
human	WB,IHC	Mol Carcinog; STAMBPL1, transcriptionally regulated by SREBP1, promotes malignant behaviors of hepatocellular carcinoma cells via Wnt/β-catenin signaling pathway; Authors - Junyi Jin
human	WB	Transl Oncol; STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression; Authors - Xiang Yang
human,mouse	WB,IF,CoIP	Cell Rep; A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma; Authors - Hongli Zhang ; KO Validated

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