Anticorps Monoclonal anti-STAR

STAR Monoclonal Antibody for WB, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G6E6

N° de cat : 67130-1-PBS

Synonymes

1G6E6, STARD1, START domain-containing protein 1

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Galerie de données de validation

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  WB analysis of pig adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  WB analysis using 67130-1-Ig (same clone as 67130-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  rat adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 67130-1-Ig (same clone as 67130-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67130-1-Ig (same clone as 67130-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67130-1-Ig (same clone as 67130-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 67130-1-Ig (same clone as 67130-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 67130-1-Ig (STAR antibody) at dilution of 1:400, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and CoraLite® Plus 488 CL488-13720 (BOULE antibody, green). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67130-1-PBS cible STAR dans les applications de WB, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STAR Protéine recombinante Ag28698
• Nom complet: steroidogenic acute regulatory protein
• Masse moléculaire calculée: 285 aa, 32 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC010550
• Symbole du gène: STAR
• Identification du gène (NCBI): 6770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue

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