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Anticorps Polyclonal de lapin anti-STAT3

STAT3 Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

ELISA

Conjugaison

Non conjugué

N° de cat : 10025-2-AP

Synonymes

Acute phase response factor, APRF, HIES, STAT3

Galerie de données de validation

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  • Western Blot (WB) analysis of HEK-293 cells using STAT3 Polyclonal antibody (10025-2-AP)

    WB analysis of HEK-293 using 10025-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10025-2-AP (STAT3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT3 Polyclonal antibody (10025-2-AP)

    IHC staining of human lung cancer using 10025-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10025-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT3 Polyclonal antibody (10025-2-AP)

    IHC staining of human lung cancer using 10025-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10025-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10025-2-AP cible STAT3 dans les applications de ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène STAT3 Protéine recombinante Ag0018
Nom complet signal transducer and activator of transcription 3 (acute-phase response factor)
Masse moléculaire calculée 90-100 kDa
Numéro d’acquisition GenBankBC014482
Symbole du gène STAT3
Identification du gène (NCBI) 6774
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against human STAT3 fusion protein.