- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-STAT3
STAT3 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, rat et plus (1)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugaison
Non conjugué
CloneNo.
3G2D12
N° de cat : 60199-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HeLa, cellules A549, cellules Jurkat, cellules RAW 264.7 |
| Résultats positifs en IP | cellules HeLa |
| Résultats positifs en IHC | tissu de cancer du col de l'utérus humain, tissu cardiaque humain, tissu de cancer de l'endomètre humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu hépatique humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | cellules HepG2, |
| Résultats positifs en cytométrie | cellules HepG2, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochimie (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 2 publications below |
| WB | See 56 publications below |
| IHC | See 7 publications below |
| IF | See 11 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
60199-1-Ig cible STAT3 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat
| Réactivité | Humain, rat |
| Réactivité citée | rat, Humain, poisson-zèbre |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | STAT3 Protéine recombinante Ag0360 |
| Nom complet | signal transducer and activator of transcription 3 (acute-phase response factor) |
| Masse moléculaire calculée | 770 aa, 88 kDa |
| Poids moléculaire observé | 70-88 kDa |
| Numéro d’acquisition GenBank | BC000627 |
| Symbole du gène | STAT3 |
| Identification du gène (NCBI) | 6774 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for STAT3 antibody 60199-1-Ig | Download protocol |
| IHC protocol for STAT3 antibody 60199-1-Ig | Download protocol |
| IF protocol for STAT3 antibody 60199-1-Ig | Download protocol |
| IP protocol for STAT3 antibody 60199-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy. | ||
J Immunother Cancer Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells | ||
Theranostics Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief. | ||
Clin Transl Med PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer. | ||
J Invest Dermatol Keratinocyte growth factor-2 regulates signal-transducing adaptor protein-2-mediated signal transducer and activator of transcription 3 signaling and reduces skin scar formation. | ||
Cell Biol Toxicol Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways |
