Anticorps Recombinant de lapin anti-STAT3

STAT3 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB

Conjugaison

Non conjugué

CloneNo.

3H1

N° de cat : 80149-1-RR

Synonymes

Acute phase response factor, APRF, FLJ20882, HIES, STAT3

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Galerie de données de validation

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  WB analysis using 80149-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80149-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 80149-1-RR

  WB result of STAT3 antibody (80149-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected HeLa cells.

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80149-1-RR cible STAT3 dans les applications de WB et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 3 (acute-phase response factor)
• Masse moléculaire calculée: 770 aa, 88 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC000627
• Symbole du gène: STAT3
• Identification du gène (NCBI): 6774
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3.