Anticorps Recombinant de lapin anti-STAT3

• Phare
• Validé par KD/KO

STAT3 Recombinant Antibody for WB, IHC, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

Publications(3)

CloneNo.

3H1

Avis (1)

N° de cat : 80149-1-RR

Synonymes

3H1, Acute-phase response factor, APRF, HIES, STAT 3

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Galerie de données de validation

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  WB analysis using 80149-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 80149-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 80149-1-RR

  WB result of STAT3 antibody (80149-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected HeLa cells.

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  IHC staining of human breast cancer using 80149-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80149-1-RR (STAT3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 80149-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80149-1-RR (STAT3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  ChIP experiment of HepG2 using 80149-1-RR

  Chromatin was prepared from HepG2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of STAT3 (80149-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en ChIP-qPCR: IL-6 (100 ng/ml, 30 min) HepG2 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

80149-1-RR cible STAT3 dans les applications de WB, IHC, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 3 (acute-phase response factor)
• Masse moléculaire calculée: 770 aa, 88 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC000627
• Symbole du gène: STAT3
• Identification du gène (NCBI): 6774
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3.

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; The pterostilbene-dihydropyrazole derivative Ptd-1 ameliorates vascular calcification by regulating inflammation; Authors - Xiaoning Wei
mouse	WB	MedComm (2020); Nicotinamide mononucleotide protects STAT1 from oxidative stress-induced degradation to prevent colorectal tumorigenesis; Authors - Ting Li
mouse	WB	Int J Biol Macromol; Matrix metallopeptidase 2-responsive curcumin-loaded nanoparticles-induced signal transducer and activator of transcription 3 inhibition suppresses glioblastoma multiforme growth via enhancing nuclear factor erythroid 2-related factor 2 activity; Authors - Fujie Jia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jianhua (Verified Customer) (05-28-2025)

great results for western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human cancer cells

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