Anticorps Polyclonal de lapin anti-STAT4

• Phare
• Validé par KD/KO

STAT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 13028-1-AP

Synonymes

DPMC, SLEB11

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Galerie de données de validation

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  WB analysis using 13028-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13028-1-AP (STAT4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 13028-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13028-1-AP (STAT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13028-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13028-1-AP (STAT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13028-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13028-1-AP (STAT4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 13028-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13028-1-AP (STAT4 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 13028-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT4 antibody (13028-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules PC-3, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

13028-1-AP cible STAT4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAT4 Protéine recombinante Ag3660
• Nom complet: signal transducer and activator of transcription 4
• Masse moléculaire calculée: 748 aa, 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC031212
• Symbole du gène: STAT4
• Identification du gène (NCBI): 6775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Front Immunol; Native CGRP Neuropeptide and Its Stable Analogue SAX, But Not CGRP Peptide Fragments, Inhibit Mucosal HIV-1 Transmission.; Authors - Jammy Mariotton
rat,human	WB	Food Chem Toxicol; HDAC inhibitor HPTA initiates anti-tumor response by CXCL9/10-recruited CXCR3+CD4+T cells against PAHs carcinogenicity; Authors - Chen Chen
human	WB	Biosci Rep; Identification and validation of STAT4 as a prognostic biomarker in acute myeloid leukemia; Authors - Chenyu Li ; KD Validated
rat	WB	Iran J Basic Med Sci; Quercetin of huoxuehuayu tongluo decoction and azithromycin combination therapy effectively improves rat tubal factor infertility by inhibiting inflammation; Authors - Liang Shao
human	WB	Acta Pharmacol Sin; Bardoxolone displays potent activity against triple negative breast cancer by inhibiting the TRIP13/STAT3 circuit; Authors - Jun-Hao Deng

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