Anticorps Polyclonal de lapin anti-STAT4

STAT4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 51070-2-AP

Synonymes

SLEB11, STAT4

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Galerie de données de validation

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  WB analysis using 51070-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51070-2-AP (STAT4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51070-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51070-2-AP (STAT4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, tissu testiculaire de rat, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

51070-2-AP cible STAT4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 4
• Masse moléculaire calculée: 748 aa, 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC031212
• Symbole du gène: STAT4
• Identification du gène (NCBI): 6775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

Publications

Species	Application	Title
mouse	WB	J Immunol; Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells.; Authors - James P Hewitson
human	WB	Food Chem; Cytotoxic withanolides from the stems and leaves of Physalis ixocarpa; Authors - Ke Xiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katie (Verified Customer) (04-08-2022)

Works well for western blot. We have published with this antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary CD4 T cells