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Anticorps Monoclonal anti-STAT5A

STAT5A Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1F10F6

N° de cat : 66459-1-Ig

Synonymes

MGF, STAT5, STAT5A

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using STAT5A Monoclonal antibody (66459-1-Ig)

    WB analysis using 66459-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    WB analysis of U-937 using 66459-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    IF Staining of HepG2 using 66459-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    IF Staining of HepG2 using 66459-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Applications testées

Résultats positifs en WBcellules RAW 264.7, cellules U-937, tissu splénique de porc, tissu splénique de rat
Résultats positifs en IFcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 1 publications below

Informations sur le produit

66459-1-Ig cible STAT5A dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène STAT5A Protéine recombinante Ag19546
Nom complet signal transducer and activator of transcription 5A
Masse moléculaire calculée 794 aa, 92 kDa
Poids moléculaire observé 95 kDa
Numéro d’acquisition GenBankBC027036
Symbole du gène STAT5A
Identification du gène (NCBI) 6776
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

Protocole

Product Specific Protocols
WB protocol for STAT5A antibody 66459-1-IgDownload protocol
IF protocol for STAT5A antibody 66459-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Intrinsic RIG-I restrains STAT5 activation to modulate anti-tumor activity of CD8+ T cells

Authors - Xinyi Jiang
View Article