Anticorps Monoclonal anti-STAT6

• Phare
• Validé par KD/KO

STAT6 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

2E5B8

N° de cat : 66717-1-Ig

Synonymes

2E5B8, IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B

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Galerie de données de validation

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  WB analysis using 66717-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HeLa using 66717-1-Ig

  WB result of STAT6 antibody (66717-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected HeLa cells.

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  WB analysis using 66717-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 66717-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66717-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66717-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66717-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu d'appendicite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66717-1-Ig cible STAT6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STAT6 Protéine recombinante Ag0410
• Nom complet: signal transducer and activator of transcription 6, interleukin-4 induced
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC005823
• Symbole du gène: STAT6
• Identification du gène (NCBI): 6778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Publications

Species	Application	Title
mouse	IF	Front Immunol; PPARγ as an E3 Ubiquitin-Ligase Impedes Phosphate-Stat6 Stability and Promotes Prostaglandins E2-Mediated Inhibition of IgE Production in Asthma.; Authors - Jia Wu
human	WB	J Cell Mol Med; Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway.; Authors - Jiapeng Hu
mouse	WB	Immunol Invest; Absence of TNF Leads to Alternative Activation in Peritoneal Macrophages in Experimental Listeria Monocytogenes Infection.; Authors - Xinying Li
human	WB	J Invest Dermatol; Benvitimod Inhibits IL-4- and IL-13-Induced Tight Junction Impairment by Activating AHR/ARNT Pathway and Inhibiting STAT6 Phosphorylation in Human Keratinocytes; Authors - Xiaojie Wang
human	WB	Stem Cell Res Ther; Intranasal delivery of hMSC-derived supernatant for treatment of ischemic stroke by inhibiting the pro-inflammatory polarization of neutrophils; Authors - Yixiang Jiang
human	WB	Cancers (Basel); GATA3-Driven ceRNA Network in Lung Adenocarcinoma Bone Metastasis Progression and Therapeutic Implications; Authors - Yun Liu

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