Anticorps Recombinant de lapin anti-STAT6

• Phare
• Validé par KD/KO

STAT6 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4F19

N° de cat : 82630-1-PBS

Synonymes

4F19, IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B

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Galerie de données de validation

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  WB analysis using 82630-1-RR (same clone as 82630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82630-1-RR (STAT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82630-1-RR (same clone as 82630-1-PBS)

  WB result of STAT6 antibody (82630-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected HeLa cells. This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82630-1-RR (same clone as 82630-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT6 antibody (82630-1-RR, Clone: 4F19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82630-1-PBS cible STAT6 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 6, interleukin-4 induced
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 105-110 kDa
• Numéro d’acquisition GenBank: BC075852
• Symbole du gène: STAT6
• Identification du gène (NCBI): 6778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

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