Anticorps Recombinant de lapin anti-STAU1

• Phare
• Validé par KD/KO

STAU1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242179D12

N° de cat : 84822-4-RR

Synonymes

242179D12, Double-stranded RNA-binding protein Staufen homolog 1, STAU

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Galerie de données de validation

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  WB analysis of HepG2 using 84822-4-RR

  WB result of STAU1 antibody (84822-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAU1 transfected HepG2 cells.

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  WB analysis using 84822-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84822-4-RR (STAU1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84822-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84822-4-RR against Human STAU1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84822-4-RR cible STAU1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: STAU1 Protéine recombinante Ag5464
• Nom complet: staufen, RNA binding protein, homolog 1 (Drosophila)
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC050432
• Symbole du gène: STAU1
• Identification du gène (NCBI): 6780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.

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