Anticorps Polyclonal de lapin anti-STAU2

• Phare
• Validé par KD/KO

STAU2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15998-1-AP

Synonymes

Double-stranded RNA-binding protein Staufen homolog 2

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Galerie de données de validation

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  WB analysis of mouse heart using 15998-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15998-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15998-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15998-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15998-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15998-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15998-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15998-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15998-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15998-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15998-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15998-1-AP (STAU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 15998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15998-1-AP (STAU2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15998-1-AP (STAU2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cérébral de souris, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

15998-1-AP cible STAU2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAU2 Protéine recombinante Ag8482
• Nom complet: staufen, RNA binding protein, homolog 2 (Drosophila)
• Masse moléculaire calculée: 570 aa, 62 kDa
• Poids moléculaire observé: 62-66 kDa
• Numéro d’acquisition GenBank: BC008370
• Symbole du gène: STAU2
• Identification du gène (NCBI): 27067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
mouse,human	WB	J Biol Chem; Staufen2 dysregulation in neurodegenerative disease; Authors - Sharan Paul ; KD Validated

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