Anticorps Monoclonal anti-STBD1

STBD1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A2G2

N° de cat : 67018-1-PBS

Synonymes

1A2G2, Genethonin-1, GENX-3414, Glycophagy cargo receptor STBD1, starch binding domain 1

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Galerie de données de validation

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  WB analysis using 67018-1-Ig (same clone as 67018-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67018-1-Ig (STBD1 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67018-1-Ig (same clone as 67018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67018-1-Ig (same clone as 67018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67018-1-Ig (same clone as 67018-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STBD1 antibody (67018-1-Ig, Clone: 1A2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50130-2

  Cytometric bead array standard curve of MP50130-2, STBD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67018-1-PBS. Detection antibody: 67018-2-PBS. Standard:Ag28613. Range: 1.563-100 ng/mL.

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Informations sur le produit

67018-1-PBS cible STBD1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STBD1 Protéine recombinante Ag28613
• Nom complet: starch binding domain 1
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC022301
• Symbole du gène: STBD1
• Identification du gène (NCBI): 8987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

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