Anticorps Polyclonal de lapin anti-Stanniocalcin 2

Stanniocalcin 2 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10314-1-AP

Synonymes

stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP

---

Galerie de données de validation

• 

  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of U2OS using 10314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Stanniocalcin 2 antibody (10314-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

10314-1-AP cible Stanniocalcin 2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Stanniocalcin 2 Protéine recombinante Ag0359
• Nom complet: stanniocalcin 2
• Masse moléculaire calculée: 33 kDa
• Numéro d’acquisition GenBank: BC000658
• Symbole du gène: STC2
• Identification du gène (NCBI): 8614
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

Publications

Species	Application	Title
human	WB,IHC	Eur Urol; Identification of stanniocalcin 2 as prognostic marker in renal cell carcinoma.; Authors - Meyer Hellmuth-A HA
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi
human	IHC	Genomics Proteomics Bioinformatics; miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma.; Authors - Bo Hu
human	IHC	Int J Cancer; Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma.; Authors - Parris Toshima Z TZ
human	IHC	Endocr Relat Cancer; Tumor suppressor functions of DAXX through histone H3.3/H3K9me3 pathway in pancreatic NETs.; Authors - Hiroki Ueda
human	WB	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu