Anticorps Recombinant de lapin anti-STEAP3

STEAP3 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1N5

N° de cat : 83484-2-RR

Synonymes

hpHyde, EC:1.16.1.-, Dudulin-2, Dudulin 2, dudlin 2

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Galerie de données de validation

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  WB analysis using 83484-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83484-2-RR (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83484-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug STEAP3 Recombinant antibody (83484-2-RR, Clone:1N5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83484-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83484-2-RR against Human STEAP3 were performed. The affinity constant is 0.89 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HepG2, cellules PC-12
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83484-2-RR cible STEAP3 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: STEAP3 Protéine recombinante Ag29528
• Nom complet: STEAP family member 3
• Masse moléculaire calculée: 488 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC042150
• Symbole du gène: STEAP3
• Identification du gène (NCBI): 55240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

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