Anticorps Polyclonal de lapin anti-STIM1

• Phare
• Validé par KD/KO

STIM1 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

59

Avis (2)

N° de cat : 11565-1-AP

Synonymes

D11S4896E, GOK, STIM1, stromal interaction molecule 1

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Galerie de données de validation

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  WB analysis using 11565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11565-1-AP (STIM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HepG2 using 11565-1-AP

  1X10^6 HepG2 cells were stained with .2ug STIM1 antibody (11565-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 15 publications below
• WB: See 44 publications below
• IHC: See 8 publications below
• IF: See 18 publications below
• IP: See 3 publications below
• FC: See 3 publications below

Informations sur le produit

11565-1-AP cible STIM1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STIM1 Protéine recombinante Ag2140
• Nom complet: stromal interaction molecule 1
• Masse moléculaire calculée: 685 aa, 77 kDa
• Poids moléculaire observé: 77-90 kDa
• Numéro d’acquisition GenBank: BC021300
• Symbole du gène: STIM1
• Identification du gène (NCBI): 6786
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.

Publications

Species	Application	Title
mouse	WB	Cell Res; Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.; Authors - Ao Hu ; KO Validated
human	FC,IF	J Immunother Cancer; PD1 blockade enhances K+ channel activity, Ca2+ signaling, and migratory ability in cytotoxic T lymphocytes of patients with head and neck cancer.; Authors - Hannah S Newton
mouse	WB, IF	Proc Natl Acad Sci U S A; STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node.; Authors - Hengtao Zhang
human,mouse	WB	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach ; KD Validated
human	WB, IF	Int J Mol Sci; Expression and Localization of Thrombospondins, Plastin 3, and STIM1 in Different Cartilage Compartments of the Osteoarthritic Varus Knee.; Authors - Daniela Mählich
human	WB,IF	Oncogenesis; Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth; Authors - Chunming Gu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cells

FH

lieqi (Verified Customer) (12-19-2018)

• Applications: Western Blot,
• Cell Tissue Type: T84

STIM1 Antibody Western Blot, validation ( dilution) in T84 (Cat no:11565-1-AP)

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