Anticorps Monoclonal anti-STIM1

• Phare
• Validé par KD/KO

STIM1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D3A3

N° de cat : 66189-1-PBS

Synonymes

STRMK, stim-1, STIM 1, IMD10, GOK

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Galerie de données de validation

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  WB analysis of K-562 using 66189-1-Ig (same clone as 66189-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66189-1-Ig (same clone as 66189-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66189-1-Ig (same clone as 66189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66189-1-Ig (same clone as 66189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66189-1-PBS cible STIM1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STIM1 Protéine recombinante Ag17914
• Nom complet: stromal interaction molecule 1
• Masse moléculaire calculée: 685 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC021300
• Symbole du gène: STIM1
• Identification du gène (NCBI): 6786
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.

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