Anticorps Polyclonal de lapin anti-STIP1

• Phare
• Validé par KD/KO

STIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 15218-1-AP

Synonymes

Hop, Hsc70/Hsp90 organizing protein, Hsc70/Hsp90-organizing protein, IEF SSP 3521, P60

---

Galerie de données de validation

• 

  WB analysis using 15218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15218-1-AP (STIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15218-1-AP (STIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15218-1-AP

  WB result of STIP1 antibody (15218-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STIP1 transfected HEK-293 cells.

• 

  IP experiment of Jurkat using 15218-1-AP

  IP result of anti-STIP1 (IP:15218-1-AP, 4ug; Detection:15218-1-AP 1:500) with Jurkat cells lysate 2400ug.

• 

  IHC staining of human liver cancer using 15218-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15218-1-AP (STIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 15218-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15218-1-AP (STIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 15218-1-AP

  Immunofluorescent analysis of MCF-7 cells, using STIP1 antibody 15218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, human normal colon; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15218-1-AP cible STIP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STIP1 Protéine recombinante Ag7388
• Nom complet: stress-induced-phosphoprotein 1
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC002987
• Symbole du gène: STIP1
• Identification du gène (NCBI): 10963
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).

Publications

Species	Application	Title
human	WB	Nature; Structure of Hsp90-Hsp70-Hop-GR reveals the Hsp90 client-loading mechanism.; Authors - Ray Yu-Ruei Wang
human	WB	Cancer Manag Res; STIP1 Regulates Proliferation and Migration of Lung Adenocarcinoma Through JAK2/STAT3 Signaling Pathway.; Authors - Xiangjun Guo ; KD Validated
human	WB	Hum Cell; Stress-induced phosphoprotein 1 facilitates breast cancer cell progression and indicates poor prognosis for breast cancer patients.; Authors - Lizhi Lin
human	WB	Mol Biol Cell; Mechanism of chaperone recruitment and retention on mitochondrial precursors; Authors - Szymon Juszkiewicz
human	WB	Gene; Systematic study of stress-inducible protein 1 (Stip1) in male reproductive system and its expression during stress response.; Authors - Yanwei Wang
mouse,human	WB,IHC,IF	Exp Cell Res; Tumor-promoting action of ubiquitin protease 43 in gastric cancer progression through deubiquitination and stabilization of stress-inducible phosphoprotein 1; Authors - Zijun Guo

{{ptg:RelatedPrimaryAntibodies}}