Anticorps Monoclonal anti-STIP1

• Phare
• Validé par KD/KO

STIP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B5H2

N° de cat : 68155-1-PBS

Synonymes

Hop, Hsc70/Hsp90 organizing protein, Hsc70/Hsp90-organizing protein, IEF SSP 3521, P60

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Galerie de données de validation

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  WB analysis using 68155-1-Ig (same clone as 68155-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68155-1-Ig (STIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68155-1-Ig (same clone as 68155-1-PBS)

  WB result of STIP1 antibody (68155-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STIP1 transfected HEK-293 cells. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

  IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 68155-1-Ig (same clone as 68155-1-PBS)

  IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with Jurkat cells lysate 2400 ug. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STIP1 antibody (68155-1-Ig, Clone: 1B5H2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 68155-1-Ig (same clone as 68155-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug STIP1 Monoclonal antibody (68155-1-Ig, Clone:1B5H2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68155-1-PBS cible STIP1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STIP1 Protéine recombinante Ag7851
• Nom complet: stress-induced-phosphoprotein 1
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC002987
• Symbole du gène: STIP1
• Identification du gène (NCBI): 10963
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).

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