Anticorps Monoclonal anti-STK11/LKB1

STK11/LKB1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D11E5

N° de cat : 68016-1-PBS

Synonymes

LKB1, STK11, STK11/ LKB1, PJS, Liver kinase B1

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Galerie de données de validation

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  WB analysis of HEK-293 using 68016-1-Ig (same clone as 68016-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68016-1-Ig (same clone as 68016-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 68016-1-Ig (same clone as 68016-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68016-1-Ig (same clone as 68016-1-PBS)

  Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68016-1-Ig (same clone as 68016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68016-1-Ig (same clone as 68016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 68016-1-Ig (same clone as 68016-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using STK11/LKB1 antibody (68016-1-Ig, Clone: 2D11E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 68016-1-Ig (same clone as 68016-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using STK11/LKB1 antibody (68016-1-Ig, Clone: 2D11E5 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68016-1-PBS cible STK11/LKB1 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: serine/threonine kinase 11
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC007981
• Symbole du gène: LKB1
• Identification du gène (NCBI): 6794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.

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