Anticorps Polyclonal de lapin anti-STK17B

• Phare
• Validé par KD/KO

STK17B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 26600-1-AP

Synonymes

DRAK2, serine/threonine kinase 17b, STK17B

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Galerie de données de validation

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  WB analysis of A375 using 26600-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 26600-1-AP (STK17B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26600-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26600-1-AP (STK17B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26600-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26600-1-AP (STK17B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 26600-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26600-1-AP (STK17B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 26600-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26600-1-AP (STK17B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 26600-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26600-1-AP (STK17B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 26600-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26600-1-AP (STK17B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HepG2, cellules K-562
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

26600-1-AP cible STK17B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STK17B Protéine recombinante Ag24389
• Nom complet: serine/threonine kinase 17b
• Masse moléculaire calculée: 372 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC016040
• Symbole du gène: STK17B
• Identification du gène (NCBI): 9262
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serine/threonine kinase 17B (STK17B), also named as DRAK2, whose gene is located on chromosome 2 (2q32.3), was first reported by Sanjo et al. in 1998 (PMID: 9786912). STK17B has been identified as a promising therapeutic target for type 1 diabetes, multiple sclerosis, and graft rejection. There are also some reports demonstrated that STK17B was related to apoptosis in various cell types, such as islet β-cells and acute myeloid leukemia cells. Some researches revealed that STK17B was deregulated in some cancers and have important role in cancer progression (PMID: 29445189). It has an indispensable role in NAFLD/NASH and offer a potential therapeutic arget for this disease (PMID: 34614409).

Publications

Species	Application	Title
human	IHC	Cell Metab; DRAK2 aggravates nonalcoholic fatty liver disease progression through SRSF6-associated RNA alternative splicing.; Authors - Yufeng Li ; KO Validated
human	WB	Nat Immunol; A multi-kinase inhibitor screen identifies inhibitors preserving stem-cell-like chimeric antigen receptor T cells; Authors - Feifei Song

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