Anticorps Recombinant de lapin anti-STK39

• Phare
• Validé par KD/KO

STK39 Recombinant Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

230382G9

N° de cat : 83081-2-RR

Synonymes

230382G9, DCHT, PASK, SPAK, Ste 20 related kinase

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Galerie de données de validation

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  WB analysis using 83081-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83081-2-RR (STK39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83081-2-RR

  WB result of STK39 antibody (83081-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK39 transfected HepG2 cells.

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  IF Staining of mouse brain using 83081-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 83081-2-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83081-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83081-2-RR against Human STK39 were performed. The affinity constant is 2.52 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293T, cellules HepG2, cellules MCF-7, cellules SH-SY5Y, tissu cérébral de rat
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83081-2-RR cible STK39 dans les applications de WB, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: STK39 Protéine recombinante Ag34503
• Nom complet: serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC166614
• Symbole du gène: STK39
• Identification du gène (NCBI): 27347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).

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