Anticorps Recombinant de lapin anti-STK39

STK39 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230382G12

N° de cat : 83081-3-PBS

Synonymes

230382G12, DCHT, PASK, SPAK, Ste 20 related kinase

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Galerie de données de validation

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  IHC staining of mouse brain using 83081-3-RR (same clone as 83081-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83081-3-RR (STK39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83081-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83081-3-RR (same clone as 83081-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STK39 antibody (83081-3-RR, Clone: 230382G12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83081-3-PBS in a different storage buffer formulation.

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  FC experiment of Daudi using 83081-3-RR (same clone as 83081-3-PBS)

  1x10^6 Daudi cells were intracellularly stained with 0.25 ug Anti-Human STK39 (83081-3-RR, Clone:230382G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83081-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83081-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83081-3-RR against Human STK39 were performed. The affinity constant is 1.21 nM.

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Informations sur le produit

83081-3-PBS cible STK39 dans les applications de IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: STK39 Protéine recombinante Ag34503
• Nom complet: serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
• Masse moléculaire calculée: 59 kDa
• Numéro d’acquisition GenBank: BC166614
• Symbole du gène: STK39
• Identification du gène (NCBI): 27347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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