Anticorps Polyclonal de lapin anti-STMN2

• Phare
• Validé par KD/KO

STMN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(37)

Avis (1)

N° de cat : 10586-1-AP

Synonymes

SCG 10, SCG10, SCGN 10, SCGN10, SGC 10

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Galerie de données de validation

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  WB analysis using 10586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10586-1-AP (STMN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10586-1-AP

  WB result of STMN2 antibody (10586-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STMN2 transfected SH-SY5Y cells.

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  WB analysis using 10586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10586-1-AP (STMN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10586-1-AP

  IP result of anti-STMN2 (IP:10586-1-AP, 4ug; Detection:10586-1-AP 1:10000) with mouse brain tissue lysate 1840 ug.

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  IHC staining of mouse brain using 10586-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10586-1-AP (STMN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10586-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10586-1-AP (STMN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 10586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (10586-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Neuro-2a, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 17 publications below

Informations sur le produit

10586-1-AP cible STMN2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STMN2 Protéine recombinante Ag0929
• Nom complet: stathmin-like 2
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 18-21 kDa
• Numéro d’acquisition GenBank: BC006302
• Symbole du gène: STMN2
• Identification du gène (NCBI): 11075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

Publications

Species	Application	Title
mouse	WB	Neuron; Molecular substrates of altered axonal growth and brain connectivity in a mouse model of schizophrenia.; Authors - Jun Mukai
Human	WB	Autophagy; Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations.; Authors - Hongyu Li
mouse	WB	Proc Natl Acad Sci U S A; SCG10 is a JNK target in the axonal degeneration pathway.; Authors - Shin Jung Eun JE ; KD Validated
mouse	WB	J Cell Biol; SARM1 acts downstream of neuroinflammatory and necroptotic signaling to induce axon degeneration.; Authors - Kwang Woo Ko
mouse	IF	Cell Rep; Comparative transcriptomic profiling reveals a role for Olig1 in promoting axon regeneration; Authors - Xiu-Qing Fu
mouse	WB	J Cell Biol; A neuroprotective agent that inactivates prodegenerative TrkA and preserves mitochondria.; Authors - Konstantin Feinberg

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (01-09-2023)

Good signal both in WB and ICC on neuroblastoma cells (neuronal cell line). Was used 1:1000 in both applications. Gave some non-specific bands in WB. ICC - 5% goat serum / PBST WB - 4% milk / TBST

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

STMN2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:10586-1-AP)

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