Anticorps Polyclonal de lapin anti-STMN2

• Phare
• Validé par KD/KO

STMN2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 10586-1-AP

Synonymes

Protein SCG10, SCG10, SCGN10, SGC10, Stathmin 2, stathmin like 2, STMN2

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Galerie de données de validation

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  WB analysis using 10586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10586-1-AP (STMN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10586-1-AP

  IP result of anti-STMN2(IP:10586-1-AP, 4ug; Detection:10586-1-AP 1:2000) with mouse brain tissue lysate 2080 ug.

• 

  IHC staining of mouse brain using 10586-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10586-1-AP (STMN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10586-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10586-1-AP (STMN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 10586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (10586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

10586-1-AP cible STMN2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STMN2 Protéine recombinante Ag0929
• Nom complet: stathmin-like 2
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 18-21 kDa
• Numéro d’acquisition GenBank: BC006302
• Symbole du gène: STMN2
• Identification du gène (NCBI): 11075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

Publications

Species	Application	Title
mouse	WB	Neuron; Molecular substrates of altered axonal growth and brain connectivity in a mouse model of schizophrenia.; Authors - Jun Mukai
mouse	WB	Proc Natl Acad Sci U S A; SCG10 is a JNK target in the axonal degeneration pathway.; Authors - Shin Jung Eun JE ; KD Validated
mouse	WB	J Cell Biol; SARM1 acts downstream of neuroinflammatory and necroptotic signaling to induce axon degeneration.; Authors - Kwang Woo Ko
mouse	WB	J Cell Biol; A neuroprotective agent that inactivates prodegenerative TrkA and preserves mitochondria.; Authors - Konstantin Feinberg
zebrafish	IHC	Elife; Retrograde Ret signaling controls sensory pioneer axon outgrowth.; Authors - Adam Tuttle
mouse	IHC,IF	J Neurosci; CNGA3: a target of spinal nitric oxide/cGMP signaling and modulator of inflammatory pain hypersensitivity.; Authors - Heine Sandra S

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (01-09-2023)

Good signal both in WB and ICC on neuroblastoma cells (neuronal cell line). Was used 1:1000 in both applications. Gave some non-specific bands in WB. ICC - 5% goat serum / PBST WB - 4% milk / TBST

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

STMN2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:10586-1-AP)

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