Anticorps Monoclonal anti-STMN2

• Phare
• Validé par KD/KO

STMN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1F6C4

Avis (1)

N° de cat : 67204-1-Ig

Synonymes

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

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Galerie de données de validation

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  WB analysis of PC-12 using 67204-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 67204-1-Ig

  WB result of STMN2 antibody (67204-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STMN2 transfected SH-SY5Y cells.

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  WB analysis of fetal human brain using 67204-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67204-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67204-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67204-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67204-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67204-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 67204-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (67204-1-Ig, Clone: 1F6C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules SH-SY5Y, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

67204-1-Ig cible STMN2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STMN2 Protéine recombinante Ag28990
• Nom complet: stathmin-like 2
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC006302
• Symbole du gène: STMN2
• Identification du gène (NCBI): 11075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.

Publications

Species	Application	Title
		Neuron; Molecular pathways and diagnosis in spatially resolved Alzheimer's hippocampal atlas; Authors - Pan Wang
human	IF	Nature; A model of human neural networks reveals NPTX2 pathology in ALS and FTLD; Authors - Marian Hruska-Plochan
human	IF	Neuron; TDP-43 seeding induces cytoplasmic aggregation heterogeneity and nuclear loss of function of TDP-43; Authors - Jens Rummens
human	WB	bioRxiv; Tubulin Regulates the Stability and Localization of STMN2 by Binding Preferentially to Its Soluble Form; Authors - Xiang Deng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (06-15-2025)

Incubation ON at 4C, in 5% goat serum/PBST. Gives specific cytoplasmic/Golgi signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cells/human

STMN2 Antibody Immunofluorescence validation (1:1000 dilution) in SH-SY5Y cells/human (Cat no:67204-1-Ig)

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