Anticorps Monoclonal anti-STMN2

• Phare
• Validé par KD/KO

STMN2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F6C4

N° de cat : 67204-1-PBS

Synonymes

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

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Galerie de données de validation

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  WB analysis of PC-12 using 67204-1-Ig (same clone as 67204-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 67204-1-Ig (same clone as 67204-1-PBS)

  WB result of STMN2 antibody (67204-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STMN2 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 67204-1-Ig (same clone as 67204-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67204-1-Ig (same clone as 67204-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67204-1-Ig (same clone as 67204-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67204-1-Ig (same clone as 67204-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67204-1-Ig (same clone as 67204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67204-1-Ig (same clone as 67204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 67204-1-Ig (same clone as 67204-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (67204-1-Ig, Clone: 1F6C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67204-1-PBS cible STMN2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STMN2 Protéine recombinante Ag28990
• Nom complet: stathmin-like 2
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC006302
• Symbole du gène: STMN2
• Identification du gène (NCBI): 11075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.

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