Anticorps Monoclonal anti-STOML2

• Phare
• Validé par KD/KO

STOML2 Monoclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1A2E9

N° de cat : 60052-1-Ig

Synonymes

EPB72 like protein 2, HSPC108, SLP 2, SLP2, stomatin (EPB72) like 2, Stomatin like protein 2, STOML2

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Galerie de données de validation

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  WB analysis of pig brain using 60052-1-Ig

  Pig, rat, and mouse brain tissues were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60052-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60052-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 60052-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 60052-1-Ig

  IP Result of anti-STOML2 (IP:60052-1-Ig, 4ug; Detection:60052-1-Ig 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 60052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules HepG2, cellules MCF-7, cellules RAW 264.7
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer de l'endomètre humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

60052-1-Ig cible STOML2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STOML2 Protéine recombinante Ag0363
• Nom complet: stomatin (EPB72)-like 2
• Masse moléculaire calculée: 356 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC002442
• Symbole du gène: STOML2
• Identification du gène (NCBI): 30968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.

Publications

Species	Application	Title
human	WB	Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima ; KD Validated
human	WB	Mol Cell Proteomics; An informatics-assisted label-free approach for personalized tissue membrane proteomics: case study on colorectal cancer.; Authors - Han Chia-Li CL
human,mouse	IF,IP	Int J Mol Sci; The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.; Authors - Meiyu Cheng
human	IHC	Int J Cancer; Novel prognostic protein markers of resectable pancreatic cancer identified by coupled shotgun and targeted proteomics using formalin-fixed paraffin-embedded tissues.; Authors - Takadate Tatsuyuki T
human	WB	Cell Death Dis; Stomatin-like protein 2 regulates survivin expression in non-small cell lung cancer cells through β-catenin signaling pathway.; Authors - Cheng-Ta Yang ; KD Validated
human	WB	J Cell Sci; Deficiency of PHB complex impairs respiratory supercomplex formation and activates mitochondrial flashes.; Authors - Chongshu Jian ; KD Validated