Anticorps Polyclonal de lapin anti-STRN

• Phare
• Validé par KD/KO

STRN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 21624-1-AP

Synonymes

SG2NA, Striatin, STRN

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Galerie de données de validation

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  WB analysis using 21624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21624-1-AP (STRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21624-1-AP (STRN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21624-1-AP (STRN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21624-1-AP

  IP result of anti-STRN(IP:21624-1-AP, 4ug; Detection:21624-1-AP 1:4000) with mouse brain tissue lysate 1080 ug.

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  IHC staining of mouse brain using 21624-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21624-1-AP (STRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 21624-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21624-1-AP (STRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 21624-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21624-1-AP (STRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21624-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21624-1-AP (STRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21624-1-AP

  Immunofluorescent analysis of Hela cells, using STRN antibody 21624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 21624-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STRN (21624-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

21624-1-AP cible STRN dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STRN Protéine recombinante Ag15984
• Nom complet: striatin, calmodulin binding protein
• Masse moléculaire calculée: 780 aa, 86 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC106879
• Symbole du gène: STRN
• Identification du gène (NCBI): 6801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STRN (Striatin), a 780-amino acid protein, was identified and cloned more than a decade ago. In adult mammals, striatin is mainly expressed in neurons of both the central and peripheral nervous systems with very high levels of expression in the striatum, hence its name. STRN-ALK fusion may be a potential therapeutic target in the aggressive forms of thyroid cancer (PMID: 24475247).

Publications

Species	Application	Title
mouse	WB,IF	Front Cell Dev Biol; Striatin Is Required for Hearing and Affects Inner Hair Cells and Ribbon Synapses.; Authors - Prathamesh T Nadar-Ponniah ; KO Validated
human	WB	J Biol Chem; Heavy Metal-Induced Metallothionein Expression is Regulated by Specific Protein Phosphatase 2A Complexes.; Authors - Liping Chen
rat	WB	Sci Rep; Precise and systematic survey of the efficacy of multicomponent drugs against functional dyspepsia.; Authors - Junying Wei
human	WB,IHC	Biomed Res Int; High STRN Expression Promotes HCC Invasion and Migration but Not Cell Proliferation or Apoptosis through Facilitating Epithelial-Mesenchymal Transition.; Authors - Qian-Yu Du ; KD Validated
rat	WB	Genes (Basel); Effect of Combined Methamphetamine and Oxycodone Use on the Synaptic Proteome in an In Vitro Model of Polysubstance Use; Authors - Daniel Meyer
human,mouse	WB	Kaohsiung J Med Sci; CircTNPO3 promotes hepatocellular carcinoma progression by sponging miR-199b-5p and regulating STRN expression; Authors - Jing Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vicki (Verified Customer) (02-21-2022)

Great antibody for cell lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary rat neurons