Anticorps Polyclonal de lapin anti-STRN3

STRN3 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 23966-1-AP

Synonymes

Cell cycle autoantigen SG2NA, GS2NA, S/G2 antigen, SG2NA, Striatin 3, STRN3

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Galerie de données de validation

• 

  WB analysis using 23966-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23966-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of MCF-7 using 23966-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 23966-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23966-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23966-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23966-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23966-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23966-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23966-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

23966-1-AP cible STRN3 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STRN3 Protéine recombinante Ag21091
• Nom complet: striatin, calmodulin binding protein 3
• Masse moléculaire calculée: 797 aa, 87 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC126221
• Symbole du gène: STRN3
• Identification du gène (NCBI): 29966
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Mol Biol Cell; Spatial proximity of proteins surrounding zyxin under force-bearing conditions.; Authors - Joleen S Cheah
human	WB,IF	STAR Protoc; Protocol using lentivirus to establish THP-1 suspension cell lines for immunostaining and confocal microscopy; Authors - Wangsheng Ji
human	WB	Genes Genomics; LncRNA KTN1-AS1 facilitates esophageal squamous cell carcinoma progression via miR-885-5p/STRN3 axis; Authors - Liying Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

Works well with Western Blot but requires long exposure >100s. Some nonspecific band but still not dirty.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

STRN3 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:23966-1-AP)