Anticorps Polyclonal de lapin anti-STT3A

• Phare
• Validé par KD/KO

STT3A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (2)

N° de cat : 12034-1-AP

Synonymes

B5, Integral membrane protein 1, ITM1, STT3 A, STT3A, TMC, Transmembrane protein TMC

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Galerie de données de validation

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  WB analysis of HepG2 using 12034-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12034-1-AP (STT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12034-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12034-1-AP (STT3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12034-1-AP cible STT3A dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STT3A Protéine recombinante Ag2698
• Nom complet: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
• Masse moléculaire calculée: 705 aa, 81 kDa
• Poids moléculaire observé: 65-100 kDa
• Numéro d’acquisition GenBank: BC020965
• Symbole du gène: STT3A
• Identification du gène (NCBI): 3703
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STT3A, also named as Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, is a 705 amino acid prtein, which belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.

Publications

Species	Application	Title
human	WB,IF,IP	Cancer Discov; Pharmacologic Suppression of B7-H4 Glycosylation Restores Antitumor Immunity in Immune-Cold Breast Cancers.; Authors - Xinxin Song ; KD Validated
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
human	WB,CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
mouse	WB	Cell Mol Gastroenterol Hepatol; Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis.; Authors - Xiaoyue Cao
human	WB	MBio; Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.; Authors - David L Lin
human	WB	EBioMedicine; Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro.; Authors - Hsiang-Chi Huang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (09-20-2023)

Good antibody for Western.

• Applications: Western Blot

FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works well

• Applications: Western Blot