Anticorps Monoclonal anti-STT3A

• Phare
• Validé par KD/KO

STT3A Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E2B12

N° de cat : 66581-1-PBS

Synonymes

ITM1, Integral membrane protein 1, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, B5

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Galerie de données de validation

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  WB analysis of THP-1 using 66581-1-Ig (same clone as 66581-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66581-1-Ig (STT3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66581-1-Ig (same clone as 66581-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66581-1-Ig (STT3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66581-1-Ig (same clone as 66581-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66581-1-Ig (STT3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66581-1-PBS cible STT3A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STT3A Protéine recombinante Ag27072
• Nom complet: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
• Masse moléculaire calculée: 705 aa, 81 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC020965
• Symbole du gène: STT3A
• Identification du gène (NCBI): 3703
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

STT3A, also named as Integral membrane protein 1, belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. There are two isoforms of STT3A with molecular weight of 81 and 70 kDa. 66581-1-Ig antibody detects a protein around 65-70 kDa in SDS-PAGE which is similar to papers published. (PMID: 32005703, 25135935)

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