Anticorps Polyclonal de lapin anti-STUB1

• Phare
• Validé par KD/KO

STUB1 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 55430-1-AP

Synonymes

Antigen NY CO 7, CHIP, CLL associated antigen KW 8, HSPABP2, NY CO 7, SDCCAG7, STUB1, UBOX1

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Galerie de données de validation

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  WB analysis of HeLa cells using 55430-1-AP

  WB result of STUB1 antibody (55430-1-AP, 1:500) with si-control and si-STUB1 transfected HeLa cells.

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  WB analysis of HEK-293 using 55430-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55430-1-AP (STUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55430-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55430-1-AP (STUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55430-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55430-1-AP (STUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55430-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55430-1-AP (STUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55430-1-AP (STUB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55430-1-AP (STUB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 55430-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 55430-1-AP (STUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Informations sur le produit

55430-1-AP cible STUB1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: STIP1 homology and U-box containing protein 1
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_005861
• Symbole du gène: STUB1
• Identification du gène (NCBI): 10273
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.

Publications

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
human	WB	Nat Commun; The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.; Authors - Zhe Li
human	IF	Nat Commun; Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.; Authors - Thomas G Martin
human	WB	Mol Cancer; Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.; Authors - Mengyan Xie
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	WB	Oncogene; AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression; Authors - Sicheng Wan

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