Anticorps Recombinant de lapin anti-Syntaxin 12

Syntaxin 12 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242546H2

N° de cat : 85069-4-PBS

Synonymes

242546H2, STX12, Syntaxin-12

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Galerie de données de validation

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  WB analysis using 85069-4-RR (same clone as 85069-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85069-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 85069-4-RR (same clone as 85069-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85069-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 85069-4-RR (same clone as 85069-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Syntaxin 12 antibody (85069-4-RR, Clone: 242546H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85069-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85069-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85069-4-RR against Human Syntaxin 12 were performed. The affinity constant is 1.05 nM.

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Informations sur le produit

85069-4-PBS cible Syntaxin 12 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Syntaxin 12 Protéine recombinante Ag5532
• Nom complet: syntaxin 12
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC046999
• Symbole du gène: Syntaxin 12
• Identification du gène (NCBI): 23673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).

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