Anticorps Recombinant de lapin anti-STX17

• Phare
• Validé par KD/KO

STX17 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4G3

N° de cat : 81899-1-PBS

Synonymes

Syntaxin-17, Syntaxin 17, 4G3

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Galerie de données de validation

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  WB analysis using 81899-1-RR (same clone as 81899-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81899-1-RR (STX17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 81899-1-RR (same clone as 81899-1-PBS)

  WB result of STX17 antibody (81899-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STX17 transfected HepG2 cells. This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 81899-1-RR (same clone as 81899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 81899-1-RR (STX17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 81899-1-RR (same clone as 81899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 81899-1-RR (STX17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81899-1-RR (same clone as 81899-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STX17 antibody (81899-1-RR, Clone: 4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81899-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81899-1-RR against Human STX17 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

81899-1-PBS cible STX17 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: STX17 Protéine recombinante Ag12134
• Nom complet: syntaxin 17
• Masse moléculaire calculée: 302 aa, 33 kDa
• Poids moléculaire observé: 33-35 kD
• Numéro d’acquisition GenBank: BC111009
• Symbole du gène: STX17
• Identification du gène (NCBI): 55014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for the fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.

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