Anticorps Recombinant de lapin anti-STX1A

STX1A Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

230470F5

N° de cat : 83159-6-PBS

Synonymes

Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1

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Galerie de données de validation

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  WB analysis using 83159-6-RR (same clone as 83159-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83159-6-RR (STX1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 83159-6-RR (same clone as 83159-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using STX1A antibody (83159-6-RR, Clone: 230470F5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

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Informations sur le produit

83159-6-PBS cible STX1A dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: syntaxin 1A (brain)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: NM_004603
• Symbole du gène: STX1A
• Identification du gène (NCBI): 6804
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A (STX1A) and 1B (STX1B), two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody recognizes human STX1A.

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