Anticorps Polyclonal de lapin anti-Syntaxin 3

Syntaxin 3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 15556-1-AP

Synonymes

STX3, STX3A, Syntaxin-3

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 15556-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 15556-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 15556-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 15556-1-AP

  IP result of anti-Syntaxin 3 (IP:15556-1-AP, 4ug; Detection:15556-1-AP 1:1000) with SH-SY5Y cells lysate 3600ug.

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  IHC staining of human kidney using 15556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Neuro-2a, tissu plasmatique humain
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 8 publications below
• IP: See 3 publications below

Informations sur le produit

15556-1-AP cible Syntaxin 3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syntaxin 3 Protéine recombinante Ag7925
• Nom complet: syntaxin 3
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC007405
• Symbole du gène: STX3
• Identification du gène (NCBI): 6809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

Publications

Species	Application	Title
mouse	WB,IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
human	WB,IP	Mol Ther; circMRPS35 promotes malignant progression and cisplatin resistance in hepatocellular carcinoma; Authors - Peng Li
mouse	IF	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
mouse	IHC	Hum Mol Genet; Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome.; Authors - Ying Hsu
mouse	IF	Front Mol Neurosci; Farnesylation of the Transducin G Protein Gamma Subunit Is a Prerequisite for Its Ciliary Targeting in Rod Photoreceptors.; Authors - Celine Brooks
human	WB	J Biol Chem; Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.; Authors - Wulin Aerbajinai

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