Anticorps Monoclonal anti-STX3

STX3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1B9C7

N° de cat : 66760-1-Ig

Synonymes

STX3, STX3A, syntaxin 3

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Galerie de données de validation

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  WB analysis using 66760-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66760-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66760-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66760-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 66760-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules NIH/3T3, cellules RAW 264.7, cellules SH-SY5Y, tissu cérébral de rat
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

66760-1-Ig cible STX3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STX3 Protéine recombinante Ag7805
• Nom complet: syntaxin 3
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC007405
• Symbole du gène: STX3
• Identification du gène (NCBI): 6809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

Publications

Species	Application	Title
human	IHC,WB	Cancer Med; Increased STX3 transcript and protein levels were associated with poor prognosis in two independent cohorts of esophageal squamous cell carcinoma patients; Authors - Takahiro Shinozuka

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