Anticorps Polyclonal de lapin anti-Syntaxin 7

• Phare
• Validé par KD/KO

Syntaxin 7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 12322-1-AP

Synonymes

STX7, syntaxin 7

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Galerie de données de validation

• 

  WB analysis of MDCK cells using 12322-1-AP

  siRNA result of 12322-1-AP (anti-STX7) by Dr.Kazunari Yamashita.

• 

  WB analysis of mouse spleen using 12322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12322-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12322-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12322-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 12322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12322-1-AP

  IP Result of anti-Syntaxin 7 (IP:12322-1-AP, 3ug; Detection:12322-1-AP 1:800) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human medulloblastoma using 12322-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human medulloblastoma using 12322-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 12322-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 12322-1-AP (Syntaxin 7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules MDCK
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de médulloblastome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

12322-1-AP cible Syntaxin 7 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syntaxin 7 Protéine recombinante Ag2975
• Nom complet: syntaxin 7
• Masse moléculaire calculée: 261 aa, 30 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC011975
• Symbole du gène: STX7
• Identification du gène (NCBI): 8417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A; Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.; Authors - Bin Na-Ryum NR
rat	WB,CoIP	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li ; KD Validated
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik
human	WB	Cell Logist; Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins.; Authors - Carolina G Ortiz-Sandoval
human,mouse	WB,IF	Neurobiol Dis; Syntaxin 7 modulates seizure activity in epilepsy; Authors - Junhong Wu
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christian (Verified Customer) (10-12-2020)

The signal is strong and clean

• Applications: Western Blot
• Cell Tissue Type: b16f10