Anticorps Polyclonal de lapin anti-STXBP1

• Phare
• Validé par KD/KO

STXBP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11459-1-AP

Synonymes

EIEE4, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

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Galerie de données de validation

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  WB analysis using 11459-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11459-1-AP

  WB result of STXBP1 antibody (11459-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STXBP1 transfected HeLa cells.

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  WB analysis of human brain using 11459-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11459-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11459-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 11459-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11459-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11459-1-AP

  IP Result of anti-STXBP1 (IP:11459-1-AP, 4ug; Detection:11459-1-AP 1:1000) with HeLa cells lysate 2400ug.

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  IHC staining of human brain using 11459-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11459-1-AP (STXBP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 11459-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11459-1-AP (STXBP1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A375 using 11459-1-AP

  Immunofluorescent analysis of A375 cells using 11459-1-AP (STXBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules A549, cellules Jurkat, cellules SH-SY5Y, cellules Y79, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

11459-1-AP cible STXBP1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STXBP1 Protéine recombinante Ag1978
• Nom complet: syntaxin binding protein 1
• Masse moléculaire calculée: 594 aa, 68 kDa
• Poids moléculaire observé: 65-68 kDa
• Numéro d’acquisition GenBank: BC015749
• Symbole du gène: STXBP1
• Identification du gène (NCBI): 6812
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB, IHC	EMBO J; Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.; Authors - Xianping Wang
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
rat	WB	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan
mouse	WB, IHC	PLoS One; Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.; Authors - Poppy Datta
mouse	WB	Cell Rep; Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies; Authors - Nadeem Murtaza