Anticorps Monoclonal anti-STXBP2

STXBP2 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

3G3A12

N° de cat : 66238-1-Ig

Synonymes

Hunc18b, MUNC18 2, pp10122, STXBP2, STXBP2 protein, syntaxin binding protein 2, UNC18 2, UNC18B

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Galerie de données de validation

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  WB analysis of K-562 using 66238-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66238-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 66238-1-Ig

  IP Result of anti-STXBP2 (IP:66238-1-Ig, 5ug; Detection:66238-1-Ig 1:2000) with K-562 cells lysate 3200ug.

• 

  IHC staining of mouse brain using 66238-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66238-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66238-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66238-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66238-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66238-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66238-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66238-1-Ig(STXBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu cérébral humain fœtal
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66238-1-Ig cible STXBP2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STXBP2 Protéine recombinante Ag7640
• Nom complet: syntaxin binding protein 2
• Masse moléculaire calculée: 593 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC002869
• Symbole du gène: STXBP2
• Identification du gène (NCBI): 6813
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.

Publications

Species	Application	Title
human		J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
human,mouse	WB,IP	Immunity; STING activation in platelets aggravates septic thrombosis by enhancing platelet activation and granule secretion; Authors - Mina Yang

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